ID31773467
Published Date2020-01-01
JournalMolecular and cellular biochemistry, 2020-01-01, Volume 464 Find other publications in this journal
Author Info
  • Department of Pharmaceutical and Administrative Sciences, University of Charleston School of Pharmacy, 2300 MacCorkle Ave SE, Charleston, WV, 25304, USA. aymenshatnawi@ucwv.edu.
  • Department of Mathematics and Computer Sciences, West Virginia State University, W729, Wallace Hall, Institute, WV, 25112, USA.
  • Department of Pharmaceutical and Administrative Sciences, University of Charleston School of Pharmacy, 2300 MacCorkle Ave SE, Charleston, WV, 25304, USA.
  • Department of Orthopedic Surgery, Baylor College of Medicine, Houston, TX, 77030, USA.

Abstract

INhibitor of Growth protein 4 (ING4) is a potential chromatin modifier that has been implicated in several cancer-related processes. However, the role of ING4 in prostate cancer (PC) is largely unknown. This study aimed to assess ING4's role in global transcriptional regulation in PC cells to identify potential cellular processes associated with ING4 loss. RNA-Seq using next-generation sequencing (NGS) was used to identify altered genes in LNCaP PC cells following ING4 depletion. Ingenuity pathways analysis (IPA) was applied to the data to highlight candidates, ING4-regulated pathways, networks and cellular processes. Selected genes were validated using RT-qPCR. RNA-Seq of LNCaP cells revealed a total of 159 differentially expressed genes (fold change ≥ 1.5 or ≤ - 1.5, FDR ≤ 0.05) following ING4 knockdown. RT-qPCR used to validate the expression level of selected genes was in agreement with RNA-Seq results. Key genes, unique pathways, and biological networks were identified using IPA analysis. This is the first report of global gene regulation in PC cells by ING4. The resultant differential expression profile revealed the potential role of ING4 in PC pathogenesis possibly through modulation of key genes, pathways and biological networks that are central drivers of the disease. Collectively, these findings shed light on a novel transcriptional regulator of PC that ultimately may influence the disease progression and as a potential target in the disease therapy.

Related Grants

31 related grants.

Sign in to view

Related Patents

1 related patents.

Sign in to view

Wellspring Search is the world's most comprehensive collection of enriched content about emerging and licensable technology innovations.


Mesh Terms

  • Cell Cycle Proteins/biosynthesis
  • Gene Expression Regulation, Neoplastic
  • Homeodomain Proteins/biosynthesis
  • Prostatic Neoplasms/metabolism
  • Tumor Suppressor Proteins/biosynthesis